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Selection, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A) Viral RNA quantification in the brain. B) Heatmap showing the abundance profiles of proteins significantly altered in brain proteomics data (limma, adjusted p < 0.05) in mock versus infected samples at any of the six time points. Column annotations represent the experimental group and time point. Rows are proteins and are annotated with log2 fold-change values from differential abundance analysis at each time point. Cells corresponding to non-significant changes are shown as empty. Selected biologically relevant proteins are labeled. C ) Volcano plots of differential protein abundance at 6 dpi compared with mock samples. Key proteins are labeled. Non-significant proteins (adjusted p ≥ 0.05) are shown in grey. D ) Western blot analysis of JEV-NS1, GOT2 and ISG15 along with the HistineH3 as loading control. E ) Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and <t>IBA1</t> (cyan). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions. F. Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and GOT2 (green). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions.
Microglia Antibody Iba1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microglia antibody iba1 - by Bioz Stars, 2026-11
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A) Viral RNA quantification in the brain. B) Heatmap showing the abundance profiles of proteins significantly altered in brain proteomics data (limma, adjusted p < 0.05) in mock versus infected samples at any of the six time points. Column annotations represent the experimental group and time point. Rows are proteins and are annotated with log2 fold-change values from differential abundance analysis at each time point. Cells corresponding to non-significant changes are shown as empty. Selected biologically relevant proteins are labeled. C ) Volcano plots of differential protein abundance at 6 dpi compared with mock samples. Key proteins are labeled. Non-significant proteins (adjusted p ≥ 0.05) are shown in grey. D ) Western blot analysis of JEV-NS1, GOT2 and ISG15 along with the HistineH3 as loading control. E ) Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and <t>IBA1</t> (cyan). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions. F. Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and GOT2 (green). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions.
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sirna transfection bv2 microglia cells  (AcceGen Biotechnology)
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A) Viral RNA quantification in the brain. B) Heatmap showing the abundance profiles of proteins significantly altered in brain proteomics data (limma, adjusted p < 0.05) in mock versus infected samples at any of the six time points. Column annotations represent the experimental group and time point. Rows are proteins and are annotated with log2 fold-change values from differential abundance analysis at each time point. Cells corresponding to non-significant changes are shown as empty. Selected biologically relevant proteins are labeled. C ) Volcano plots of differential protein abundance at 6 dpi compared with mock samples. Key proteins are labeled. Non-significant proteins (adjusted p ≥ 0.05) are shown in grey. D ) Western blot analysis of JEV-NS1, GOT2 and ISG15 along with the HistineH3 as loading control. E ) Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and <t>IBA1</t> (cyan). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions. F. Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and GOT2 (green). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions.
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murine microglia cell line sim a9  (ATCC)
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A) Viral RNA quantification in the brain. B) Heatmap showing the abundance profiles of proteins significantly altered in brain proteomics data (limma, adjusted p < 0.05) in mock versus infected samples at any of the six time points. Column annotations represent the experimental group and time point. Rows are proteins and are annotated with log2 fold-change values from differential abundance analysis at each time point. Cells corresponding to non-significant changes are shown as empty. Selected biologically relevant proteins are labeled. C ) Volcano plots of differential protein abundance at 6 dpi compared with mock samples. Key proteins are labeled. Non-significant proteins (adjusted p ≥ 0.05) are shown in grey. D ) Western blot analysis of JEV-NS1, GOT2 and ISG15 along with the HistineH3 as loading control. E ) Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and <t>IBA1</t> (cyan). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions. F. Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and GOT2 (green). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions.
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mouse  (Miltenyi Biotec)
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A) Viral RNA quantification in the brain. B) Heatmap showing the abundance profiles of proteins significantly altered in brain proteomics data (limma, adjusted p < 0.05) in mock versus infected samples at any of the six time points. Column annotations represent the experimental group and time point. Rows are proteins and are annotated with log2 fold-change values from differential abundance analysis at each time point. Cells corresponding to non-significant changes are shown as empty. Selected biologically relevant proteins are labeled. C ) Volcano plots of differential protein abundance at 6 dpi compared with mock samples. Key proteins are labeled. Non-significant proteins (adjusted p ≥ 0.05) are shown in grey. D ) Western blot analysis of JEV-NS1, GOT2 and ISG15 along with the HistineH3 as loading control. E ) Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and <t>IBA1</t> (cyan). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions. F. Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and GOT2 (green). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions.
Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
mouse - by Bioz Stars, 2026-11
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Image Search Results


A) Viral RNA quantification in the brain. B) Heatmap showing the abundance profiles of proteins significantly altered in brain proteomics data (limma, adjusted p < 0.05) in mock versus infected samples at any of the six time points. Column annotations represent the experimental group and time point. Rows are proteins and are annotated with log2 fold-change values from differential abundance analysis at each time point. Cells corresponding to non-significant changes are shown as empty. Selected biologically relevant proteins are labeled. C ) Volcano plots of differential protein abundance at 6 dpi compared with mock samples. Key proteins are labeled. Non-significant proteins (adjusted p ≥ 0.05) are shown in grey. D ) Western blot analysis of JEV-NS1, GOT2 and ISG15 along with the HistineH3 as loading control. E ) Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and IBA1 (cyan). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions. F. Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and GOT2 (green). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions.

Journal: bioRxiv

Article Title: The mitochondrial transaminase GOT2 supports Japanese encephalitis virus replication in neuronal cells

doi: 10.64898/2026.10.12.758629

Figure Lengend Snippet: A) Viral RNA quantification in the brain. B) Heatmap showing the abundance profiles of proteins significantly altered in brain proteomics data (limma, adjusted p < 0.05) in mock versus infected samples at any of the six time points. Column annotations represent the experimental group and time point. Rows are proteins and are annotated with log2 fold-change values from differential abundance analysis at each time point. Cells corresponding to non-significant changes are shown as empty. Selected biologically relevant proteins are labeled. C ) Volcano plots of differential protein abundance at 6 dpi compared with mock samples. Key proteins are labeled. Non-significant proteins (adjusted p ≥ 0.05) are shown in grey. D ) Western blot analysis of JEV-NS1, GOT2 and ISG15 along with the HistineH3 as loading control. E ) Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and IBA1 (cyan). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions. F. Representative super-resolution TIRF microscopy image of infected brain tissue stained for MAP2 (red), JEV antigen (yellow) and GOT2 (green). Boxed regions i–iii are shown at higher magnification on the right. Scale bars, 50 µm in the overview image and 5 µm in magnified regions.

Article Snippet: The first cocktail included primary neuron antibody MAP2 (catalog #17490-1-AP, Proteintech) conjugated with CoraLite Plus 647 (catalog #KFA003, Proteintech) at 1:100 dilution, activated microglia antibody IBA1 (catalog #10904-1-AP, Proteintech) conjugated with CoraLite Plus 555 (catalog #KFA002, Proteintech) at 1:200 dilution, and JEV NS1 antibody (catalog #GTX633820, GneTex) conjugated with CoraLite Plus 488 (catalog #KFA521, Proteintech) at 1:500 dilution.

Techniques: Infection, Analysis, Labeling, Quantitative Proteomics, Western Blot, Control, Super Resolution, Microscopy, Tissue, Staining